FGF-2 Polyclonal Antibody
¥680.00
- 产品信息
商品描述
靶点-Target
Calregulin
反应性
Human,Mouse
应用
WB,IHC,IF,ELISA
宿主物种-Host Species
Mouse
修饰-Modification
Unmodified
MW(Calculated)
48kD
推荐稀释比
WB 1:500-1:2000;IHC 1:200-1:1000;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
Affinity purification
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
克隆性
Monoclonal
免疫原
Synthetic peptide corresponding to aa (EEEDVPGQAKDELC) of human Calreticulin, conjugated to KLH.
特异性
Calregulin Monoclonal Antibody detects endogenous levels of Calregulin protein.
基因名称
CALR
蛋白名称
Calreticulin
别名
CALR;CRTC;Calreticulin;CRP55;Calregulin;Endoplasmic reticulum resident protein 60;ERp60;HACBP;grp60
Organism-1
Human
基因ID-1
811
SwissProt-1
P27797
Organism-2
Mouse
基因ID-2
12317
SwissProt-2
P14211
背景
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its
细胞定位
Endoplasmic reticulum lumen . Cytoplasm, cytosol . Secreted, extracellular space, extracellular matrix . Cell surface . Sarcoplasmic reticulum lumen . Cytoplasmic vesicle, secretory vesicle, Cortical granule . Cytolytic granule . Also found in cell surface (T cells), cytosol and extracellular matrix (PubMed:10358038). During oocyte maturation and after parthenogenetic activation accumulates in cortical granules. In pronuclear and early cleaved embryos localizes weakly to cytoplasm around nucleus and more strongly in the region near the cortex (By similarity). In cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation (By similarity). .
