FGF-2 Polyclonal Antibody
¥680.00
DDDDK-Tag(binds to flag sequnence) rabbit pAb
活动价：
¥680.00
市场价：¥880.00
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产品规格：
- 50μL
- 100μL
货号：
FAB2600202
- 产品信息
商品描述
靶点-Target
FLAG-tag
反应性
Species independent
应用
WB,ELISA,IP,IF
宿主物种-Host Species
Rabbit
同种型-Isotype
IgG
修饰-Modification
Unmodified
推荐稀释比
WB 1:1000-1:3000;ELISA 1:5000-20000;IF 1:50-200
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
克隆性
Polyclonal
免疫原
DDDDK synthetic peptide conjugated to KLH.
特异性
FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.
基因名称
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
别名
DDDDK epitope tag;DDDDK epitope tag;DYKDDDDK epitope tag
背景
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
